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Spot Tests and Rapid Tests Q. Which indole reagent should I be using?

April 26, 2017indole rapid Reagent spot tests

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Spot Tests and Rapid Tests Q. Which indole reagent should I be using?

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A. There are three indole reagents. Kovacs Indole (Cat. no. Z67) can be used with the “tube” test or with the “spot” test for aerobic bacteria. It is the least sensitive of the three. The Indole DMACA (Cat. no. Z65) is more sensitive and can only be used with the “spot” test. It is for aerobes and anaerobes. Ehrlichs Indole is for the tube test and requires a xylene extraction. It is the most sensitive of the three (ref: Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.).