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Some of my DNA templates failed the PCR, whats wrong?

April 26, 2017dna failed PCR Templates wrong

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Some of my DNA templates failed the PCR, whats wrong?

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The most likely reason is SDS contamination of the DNA prep. If you rinse the DNA pellet with 70% ethanol from a squirt bottle, be sure to rinse the entire interior of the tube, including the cap. If the DNA pellet is large (~10 ul in size), it may be necessary to re-suspend the pellet in 50 mM sodium acetate and re-precipitate the DNA in isopropanol to completely remove any salt and detergent trapped in the pellet.