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Should I use the high copy pSMART-HCKan and -HCAmp vectors or the low copy pSMART-LCKan and -LCAmp vectors?

April 26, 2017copy high low vectors

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Should I use the high copy pSMART-HCKan and -HCAmp vectors or the low copy pSMART-LCKan and -LCAmp vectors?

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The high-copy vectors are used for most cloning and library construction projects. They have been shown to maintain large inserts (> 10kb), inserts with strong promoters, or toxic coding sequences. However, inserts with high AT content (>60-65%) may be unstable in the high copy number vector.