Should I use labeled or unlabeled duplexes for my in vivo RNAi experiments?
A. We have demonstrated that labeling Stealth RNAi™ siRNA duplexes does not hamper their knockdown potency. An alternative approach is to mix unlabeled duplexes with labeled control duplexes; this method is more commonly used with in vivo RNAi, and allows progression to clinical research unhindered by questions about the possible effects of a dye.
