Should I use enrichment media to culture milk for mycoplasma?
It all depends. Enrichment media has been shown to be useful when culturing individual cow milk samples. The recovery rate is much greater than that of the direct culture method. The lower limit of detection with enrichment is about 10 cfu and for direct plating it is about 100 cfu. The answer is not clear with bulk tank milk as there is no published research on this subject.