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Should I use chemically modified duplexes for my in vivo RNAi experiments?

April 26, 2017chemically duplexes experiments modified rnai vivo

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Should I use chemically modified duplexes for my in vivo RNAi experiments?

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A. Chemically modified Stealth RNAi™ siRNA duplexes have a number of advantages over standard RNAi duplexes, including the minimization of off-target effects, enhanced stability, and reduced toxicity. For these reasons, Stealth™ chemically modified RNAi duplexes are recommended for in vivo RNAi experiments. For some pilot experiments, where the only aim is to determine biodistribution, BLOCK-iT™ duplexes and fluorescent controls are useful and slightly more cost-effective.