Should I shake the jars colonizing with Panaeolus cyanescens?
I do not shake the jars during colonization, only to break up the block to lay out in trays. Exceptions: When a jar of substrate has uneven moisture distribution, and sections of substrate are too dry to be colonized, I shake these jars, and have only had a couple stall out, usually they rebound quickly and completely colonize. Most of the trouble with shaking occurs with multispore syringe inoculations into a jar of substrate. When you do this there is more then ONE strain or sub strain growing in the jar. When you shake, these separate strains are being mixed together, and not all of them will grow together as one. If you place spores on a petri plate, you will see there are distinct sectors growing out from the multi spore inoculation. Most of them do not interact, and actually show dieback when they come together. There is a zone of no or little growth separating each Strain or sub strain. Imagine having five or six different strains that only rarely undergo anastamosis or fusion,
