Should I add sodium azide to the antiserum?
We recommend adding sodium azide unless the serum is directly used in cell culture assays. Sodium azide prevents bacteria from growing as they can produce proteases that denature the antibodies in the serum. It can be removed by dialysis with a membrane that has a molecular weight cut-off of 13.000. Note: Sodium azide is toxic to most organisms and should be removed for some applications.