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Should anything be changed in the software when analyzing Double-Dye Oligos with quenchers other than TAMRA?

April 26, 2017analyzing changed double-dye oligos quenchers Software tamra

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Should anything be changed in the software when analyzing Double-Dye Oligos with quenchers other than TAMRA?

1 Answer

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When analysing fluorescent signals from Double-Dye Oligos labeled Dark Quenchers the background correction for TAMRA should be turned off (in plate set up window).