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Should any reagents be avoided when using of SuperSignal West Pico Substrate?

April 26, 2017avoided Pico reagents Substrate supersignal West

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Should any reagents be avoided when using of SuperSignal West Pico Substrate?

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Yes. Avoid using sodium azide during and after probing steps involving horseradish peroxidase (HRP), as this will inhibit HRP activity. Sulfide, cyanide, fluoride and superoxide ions also inhibit HRP to some extent.