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Should a pTYB vector (or a pCYB vector) be used as a control for expression?

April 26, 2017CONTROL expression pcyb ptyb Used vector

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Should a pTYB vector (or a pCYB vector) be used as a control for expression?

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pTYB1-4 vectors (or pCYB1-4 vectors) are not good controls for expression since the intact polylinker region apparently causes poor expression. The control plasmid pMYB5 (NEB#E6906, supplied in the IMPACT-CN kit) should be used as a control for expression and purification. The pTWIN-MBP1 (NEB#N6953S, supplied in the IMPACT-TWIN kit) vector an also be used as a control for expression and purification.