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Should a pTXB or a pTYB vector be used as a control for expression?

April 26, 2017CONTROL expression ptxb ptyb Used vector

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Should a pTXB or a pTYB vector be used as a control for expression?

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pTXB, pTYB vectors are not good controls for expression since the intact polylinker region apparently causes poor expression. The control plasmids pMXB10 (NEB #N6903S), pMYB5 (NEB# N6906S) or pTWIN-MBP1 (NEB#N6953S), which express maltose binding protein (MBP) as the target protein, can be used as a control for expression and purification.