Scanning Slides What type of instrument/software is required to perform the assay?
• Antibody arrays can be scanned with most commercially available ‘overhead’ lightsource DNA array scanners that accommodate standard microscope slides. • Scanners with bottom-lit light sources (e.g., Agilent) cannot be used, as the nitrocellulose coating employed with antibody arrays will interfere with the instrument’s signal detection system. • Instrument compatibility: • Standard microscope glass slide (25 mm x 75.6 mm x 1 mm) • Light filter system reading within the near-red spectrum of Cyanine dyes: Cy3 = excitation 550 nm, emission 570 nm;Cy5 = excitation 659 nm, emission 670 nm Instrument Software Perkin Elmer Packard ScanArray Lite Packard ScanArray Express Packard ScanArray Express HT ProScanArray Microarray Scanner ProScanArray HT Microarray Scanner Axon Axon 4200 AL Microarray Scanner ScanArray Express (Packard/Perkin Elmer) ProScanArray Express (Perkin Elmer) GenePix Pro 6 (Molecular Devices) ArrayPro (Media Cybernetics) Other universal software available Typical scanner s
