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Researcher cannot use the recommended Tris Buffer due to the cells. They want to know if they can use these enzymes in PBS or RPMI 1640 buffers, and if so, will there be any loss of activity?

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Researcher cannot use the recommended Tris Buffer due to the cells. They want to know if they can use these enzymes in PBS or RPMI 1640 buffers, and if so, will there be any loss of activity?

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It should be no problem to use these other buffers as long as you keep the pH around 8.0. No loss of activity is expected if pH is maintained in this level.

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