Reactivity of the Reagent FAQs How does the Fungitell assay work?
This is described in the insert. The Fungitell assay works as an amplification cascade that allows a small stimulus to produce a large output signal. (1→3)-β-D-glucan activates a protease zymogen that, in turn, activates may molecules of another protease zymogen. This protease cleaves a chromogenic substrate, producing the increase in the optical density at 405 nanometers. This amplification accounts for the extreme sensitivity of the Fungitell assay.
