PCR confirmation of a culture diagnosis was requested: how should results be reported?
Bull owners or their testing veterinarian may request PCR confirmation of a positive culture result. If confirmation by PCR has been requested, your Animal Health Branch District Office should be informed of the positive culture result, but the positive test result will not be investigated until the PCR result is reported. For the positive culture result to be reclassified as a negative official test, the PCR must be negative for T. foetus and positive for other trichomonads. CAHFS laboratories will report final test results directly to CDFA, so the testing veterinarian will not need to do so. The laboratory will report PCR results as follows: • If the bull is PCR positive, using primers specific for T. foetus , he is declared infected. • If the bull is positive for trichomonads in general, but negative for Tritrichomonas foetus, the bull is reported as negative. • If the PCR is negative for all trichomonads, it suggests that there was an insufficient amount of DNA present to be detect
Related Questions
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- PCR confirmation of a culture diagnosis was requested: how should results be reported?
