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Others I have talked to hybridize at 65 degree in the absence of formamide. Have you found better results using formamide at 42 degrees?

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Others I have talked to hybridize at 65 degree in the absence of formamide. Have you found better results using formamide at 42 degrees?

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10

No. We have experienced difficulties with probes drying out under the coverslip at high hybridization temperatures.

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