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One protein has more lysine residues. Won’t it label more than the other proteins?

April 26, 2017Label lysine protein Proteins residues

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One protein has more lysine residues. Won’t it label more than the other proteins?

1 Answer

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This might happen, but this does not affect your quantitation. The same protein will label to the same percentage with the different CyDye™ DIGE Fluor minimal dyes so your within-spot analysis is not affected. We have never seen any direct evidence of this happening.