of plastic-embedded tissue stained for light microscopy?
Answer. The wrinkles form when mounted plastic sections are stained in a hot aqueous dye solution. Chandler & Schoenwolf (1983) found that the wrinkles did not form if sections were dried down onto acid-washed slides, overnight, at 76 C. They thought acid-washing might improve the glass surface in some way. The minimum drying time was 6 hours. The temperature was also important. Variation was not fully investigated, but neither 60 C nor 90 C was efffective in preventing wrinkles. Reference: Chandler, NB & Schoenwolf, GC (1983) Wrinkle-free plastic sections for light microscopy. Stain Technology 58: 238-240. John A. Kiernan, Department of Anatomy & Cell Biology, The University of Western Ontario, London, CANADA N6A 5C1 (kiernan[AT]uwo.
Answer. The wrinkles form when mounted plastic sections are stained in a hot aqueous dye solution. Chandler & Schoenwolf (1983) found that the wrinkles did not form if sections were dried down onto acid-washed slides, overnight, at 76 C. They thought acid-washing might improve the glass surface in some way. The minimum drying time was 6 hours. The temperature was also important. Variation was not fully investigated, but neither 60 C nor 90 C was efffective in preventing wrinkles. Reference: Chandler, NB & Schoenwolf, GC (1983) Wrinkle-free plastic sections for light microscopy. Stain Technology 58: 238-240. John A.
