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My VP1.5 sequence read matches the reference but my XL39 shows no BLAST similarity to anything?

April 26, 2017blast read Reference sequence shows similarity vp1.5 xl39

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My VP1.5 sequence read matches the reference but my XL39 shows no BLAST similarity to anything?

1 Answer

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Because most of OriGene’s TrueClones contain a polyA tail, the XL39 primer can fail to read through this region accurately. In these instances, we recommend sequencing with a gene-specific forward primer to get a good 3` read. It is also possible that OriGene’s clone has a longer UTR than was present in the reference.