My samples contain beta-lactoglobulin and other milk proteins. What can I do?
Your lab probably does a lot of western blots using non-fat milk, so mush of the glass- and plastic-ware are probably contaminated with milk proteins. Although this may help you with your WBs, it makes it difficult to do mass spectrometry because the mass spectrometer will “see” peptides from all of these proteins. It is difficult to get glassware clean enough after it has been heavily saturated with protein, a simple solution is to acquire a few key pieces of equipment and reserve them for this purpose ONLY. Then NEVER expose them to milk protein!!
