My previously isolated cells, growing in my current medium did not grow very well when switched to the CELLnTEC medium. What could be the problem?
Primary cells can quickly adapt to specific cell culture media. Hence when switching a cell culture to a different medium, there is a need to wean your cells off the old medium. This is accomplished with a step-wise serial dilution of the old medium with CELLnTEC medium over a period of one to four weeks, depending on the severity of the transition and the proliferation rate of the cells.
