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My PCR machine can only be set to 100 μ L, but the MethylCode™ Bisulfite Conversion protocol recommends using 150 μ L in the conversion reaction. Is this a mistake?

bisulfite conversion Machine methylcode™ PCR Protocol recommends
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My PCR machine can only be set to 100 μ L, but the MethylCode™ Bisulfite Conversion protocol recommends using 150 μ L in the conversion reaction. Is this a mistake?

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We have found that 150 μL works fine for the conversion reaction with most PCR machines.

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