My luminometer does not have a dispenser for adding the ATP standard. What do I do?
A5: If you work with stable light emission (ATP Reagent SL) you can add the ATP standard manually and measure the cuvette or microplate again. If you work with non-stable light emission (ATP Reagent HS or SS) you can perform assays in two cuvettes or two wells – one without and one with added ATP standard. If you know that your samples have the same medium, temperature, potential luciferase inhibitors etc., you can run a limited number of samples (e.g. the growth medium) with and without ATP standard and use the ATP standard signal for all the other samples.
