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My DNA extractions always contain some degraded DNA (DNA smear). How much degradation is too much?

April 26, 2017contain degradation degraded dna Extractions smear

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My DNA extractions always contain some degraded DNA (DNA smear). How much degradation is too much?

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We do not routinely use samples in which the High Molecular Weight DNA band contains less than 50% of the total DNA. Furthermore, there is a loss of performance as the percentage of degraded DNA increases. A sample with only 50% HMW DNA will have a decreased SNP call rate (by at least 2%) when compared to a sample containing 90% HMW DNA.