My data files were not correctly labeled during collection. Can FlowJo fix the parameter labeling?
There are two different ways to annotate your data files post collection. Within FlowJo, you can double-click the parameter you would like to change (in the Workspace window) and type in the correct annotation. If the parameter you wish to alter is not displayed as a column in the Workspace, you can Edit Workspace Columns (under the Workspace menu) and add the columns that correspond to the fluorescence channels (or to any other FCS keyword). In the Workspace window you can then double click on the spreadsheet cell (i.e., the under the $P3S column across from the sample name) and type in the correct antibody. The other option is ProJo – a free FCS file editor, also made by Tree Star. ProJo reads keywords in FCS files, and displays them in an easy interface allowing you to modify their values. ProJo is particularly adept at handling group operations that alter parameters from all of the files in the group. The primary difference between FlowJo and ProJo is that ProJo makes changes in th
