My bovine sperm cells seems to be damaged by the pepsin treatment step, and possibly lysed during denaturation. What should I do?
Related Questions
- Can an individual naturally have an unequal proportion of X- and Y-bearing sperm cells even if there is a family history of mostly female or male offspring?
- My bovine sperm cells seems to be damaged by the pepsin treatment step, and possibly lysed during denaturation. What should I do?
- Should we perform a denaturation step (65C degree treatment)?