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My annealed oligos will not ligate. What is the problem?

annealed oligos problem
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My annealed oligos will not ligate. What is the problem?

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Ligation reactions require a 5´phosphate. If your oligos do not contain a 5´ phosphate, ligation will not occur or only with a very low efficiency. The problem can be addressed without ordering an additional oligo pair: phosphorylate your oligos enzymatically with kinase before use in ligation reactions.

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