Method 8310, the HPLC method for PAHs, does not list a confirmation column. What column should I use to confirm my results from the primary column?
Work at the EPA laboratory in Region III (Annapolis, MD) suggests that the following columns may be appropriate for the analysis of PAHs by HPLC: Standard analytical column – C-18 reversed-phase column, 3-5 m particle size, 100 – 250 mm x 4.6 mm ID, (Perkin-Elmer ChromSPHERE-3-PAH, Supelco Supelcosil LC-PAH, or equivalent). Narrow-bore analytical column – C-18 reversed-phase column, 3-5 m particle size, 100 – 250 mm x 2.1 mm ID, (Supelco Supelcosil LC-PAH, or equivalent). Confirmation column – CN reversed-phase column, 3-5 m particle size, 150 – 250 mm x 3.0 – 4.6 mm ID, (Supelco Supelcosil LC-PCN, Waters Nova-Pak CN HP, or equivalent). However, other HPLC columns also may be available that will provide a sufficient change in elution order to be useful for confirmation. Finally, you should consult Method 8000B for information on confirmation analyses, including when confirmations are needed and when they may not be needed.
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