May I use a cDNA clone for protein expression?
Minimal preconditions for expression of a fully functional protein are a cloning vector containing a suitable promoter for your expression system (e.g. mammalian cells, E.coli) (check the Product Data Sheet and the corresponding vector map) as well as an insert covering the complete open reading frame (ORF/cds). Please note that most of the inserts from Full Length cDNA clones comprise also long sections of the UTRs, whose influence on protein expression efficiency cannot be predicted (e.g. destabilization of mRNA, etc.). As an efficient expression using cDNA plasmids cannot be predicted reliably, we recommend to prefer our ready-to-use Full ORF Expression Clones.
