Loops Q. What is the best method of streaking, and which kind of loops are the best?
A. Avoid streaking your plates close to the outer edge. If a contaminant is present, it is most likely to be at the edge of the plate. Older loops can have a rough surface that can gouge the media. Replace your loops occasionally. Nichrome loops are less expensive than platinum and last about 80% as long. The nichrome twisted wire loops are more flexible. Another alternative is disposable plastic loops which are available in soft (flexible) and more rigid shafts. These loops are calibrated and come with a certificate of calibration and sterility.
