Limits of data file size What are the current limits on data file size when using the central Galaxy server?.
There is no restriction on the size of a data file or the amount of storage space available for each user on the central Galaxy server. However, there are practical limits. The central Galaxy server stores many alignments locally, so take advantage of them rather than attempting to retrieve them from external sources. For example, moving the entire set of 28-way alignments from UCSC to Galaxy is both time consuming and impractical. There is a much more efficient way to do this with Galaxy, as it provides access to the large alignments from UCSC through a special tool that allows exactly the intersection you want to be done efficiently: the “Fetch Alignments -> Extract MAF Blocks” tool. Simply select your interval file, along with “Locally cached alignments” and “28-way multiz”. See our MAFAnalysis page for more information on how to analyze multiple alignments through Galaxy.