I’ve altered my ES cell clone(s). Can the CMMR derive mice from my modified ES cell clone ?
To generate chimeric mice from ES cells cultured and/or manipulated at an Investigator’s lab, the clone must be submitted to the CMMR (or Transgenic Core and Specialty Resources) for clone expansion and mouse pathogen (IMPACT) testing before generation of chimeras at the TCP Transgenic Core and Specialty Resources will be attempted. Details of the ES cell clone expansion service and fees can be found on our Services and Fees pages. Mouse pathogen (IMPACT) testing is necessary to protect our clean ES cell and animal facilities from contamination. Additionally, infected ES cells perform very poorly in both aggregation and blastocyst injection and identification of these potential problems can assist the Investigator in making informed decisions. In addition to pathogen testing, the Investigator may choose to have the genomic integrity of their ES cell clone assessed either by simple chromosome counting or by full mouse karyotyping (for additional fees). Genomic assessment is optional, bu
