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It looks as if there is something insoluble at the bottom of the sample tube. What is it?

April 26, 2017insoluble looks sample tube

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It looks as if there is something insoluble at the bottom of the sample tube. What is it?

1 Answer

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Occasionally there may be some carryover of solid support to the sample tube. It appears as an insoluble, granular solid. It is chemically inert and will not affect enzyme reactions. The solid can be removed by microfuging and transferring the oligonucleotide solution to a new, sterile tube.