Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Isnt 3ml/minute too fast to get a good separation?

April 26, 2017fast good Minute Separation

0

Posted

Isnt 3ml/minute too fast to get a good separation?

1 Answer

0

Posted

Not really, while the optimum flow rate for a 4.6 mm ID column is approximately 0.8 ml/min, the Van Deemter curve is relatively flat at higher flow rates. This means that less than 40% efficiency is lost yet you will gain a threefold increase in the speed of analysis.