Is this an inherent problem with microarray analysis or proteomic analyses like mass spec?
Yes. There have been some attempts to do single-cell microarrays, but it’s very technically difficult, and essentially very little has been done. There have also been some attempts to do single-cell proteomics on very large cells, but nothing on this scale. You compared results from your flow cytometric analysis with quantitative microscopy. Could high-content single-cell imaging be used in this approach? They’re very complementary approaches. It would be very hard to beat flow on the numbers. We can count 50,000 cells in seven seconds. That would be very tough to beat. On the other hand, what you get out of microscopy is localization information, which is a very important thing. And microscopy, in a way, has traditionally been applied more to this type of approach. One of the things we were trying to point out is that flow cytometry, because of it’s really high-throughput nature, can be a nice complement by providing information that would be very hard to get using high-throughput mic
