Is there potential for false positive nucleic acid amplification test results?
OBJECTIVES: The ability of molecular methods to detect low levels of nucleic acid has lead to the widespread application of techniques based on nucleic acid amplification tests, in microbiological diagnosis. This exquisite sensitivity is recognised in the laboratory to require stringent precautions to avoid contamination, but this is not widely appreciated in clinical settings where samples are initially collected, and may be a particular problem in the non-clinical settings used for sampling as part of the National Chlamydia Screening Programme. Thus, there is the need to characterise the risk of false positive results due to environmental contamination in these areas. METHODS: The extent of environmental contamination of Chlamydia trachomatis (CT) nucleic acid in clinical settings was investigated by swabbing surfaces within the vicinity of specimen collection. Laboratory experiments were designed to monitor the persistence of ribosomal RNA (rRNA) under simulated conditions and to in
