Is there any help in understanding densitometry and/or gel analysis?
There are a number of documents available explaining anything from portions of the theoretical principles of densitometry, to step by step help in using the gel analysis macros. Those interested in densitometry should strive to understand the “Calibrate” command and why it is used. For explanation regarding basic underlying principles of densitometry read the section of Image Engineering on Fundamentals of Densitometry. For a simple explanation on the Calibrate command, see the Calibrate section of the NIH Image manual. For basic step by step explanation on the gel macros, read the Analyzing Electrophoretic Gels section of the NIH Image manual. Calibrated optical density step tablets are made by Kodak and sold through tiffen.com as described by this page: www.kodak.com/global/en/service/faqs/faq2100.shtml. How should I cite NIH Image Published research assisted by NIH Image should use a statement similar to the following in the materials and methods section “… analysis performed on a
