Is there an easy way to tell the data I submitted is pure raw data or baseline subtracted data?
A10: Yes. You can have a quick look of the data you exported from the Real-time PCR machine, especially the first 15 row. The number of pure raw data should always larger than the one after baseline subtraction. If you find any negative value or zero, that means you might export the baseline subtracted data. For the machine use the minimal value as baseline, you need choose the data set with larger value.