Is there an assay for triacylglycerol lipase ?
KIT CONTENTS (100 tests in 96-well plate) Assay Buffer (pH 8.5): 15 mL Color Reagent: 530 mg BALB Reagent: 1.0 mL Calibrator: 2.0 mL (equivalent to 735 U/L) Storage conditions. This product is shipped at room temperature. Store all components at 4°C. Shelf life of at least 6 months. Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. PROCEDURES Sample Preparation. Lipase inhibitors (EDTA, and certain detergents Tween-20, NP-40), mercaptoethanol and dithiothreitol interfere with this assay and should be avoided in sample preparation. Samples can be stored frozen for at least one month, if not assayed immediately. Tissue and cell lysates can be obtained by homogenization in cold PBS buffer and centrifugation (e.g. 5 min at 14,000 rpm). Preparation of Working Reagent. Mix Color Reagent into Assay Buffer and shake vial to mix. Add 0.8 mL BALB Reagent (sufficient for 100 assays). Alternatively for partial
