Is there a simple method to sequence lambda, M13, or plasmid clones using PCR?
PCR amplification can be can be performed using a phage plaque or bacterial colony picked directly from an agar plate. This is particularly useful for confirmation of mutants after site-directed mutagenesis, sequence tagged site(s) sequence characterization, identification of mutations following random mutagenesis, etc. For more information see Hofmann.Brian1991, Krishnan.Berg1991a, Krishnan.Berg1991b, Krishnan.Berg1993, and Wang1992. Be careful when selecting the type of toothpicks to be used because some people claim wooden toothpicks can inhibit the PCR. See Lee1995, Hengen1995Aprtibs and Hengen1997Febtibs for more details.
