Is there a preference for using EDTA or heparin in Phosflow protocols?
Generally, there is no preference between EDTA and heparin, and we do not have a specific list of which protocols are affected by the type of anti-coagulant. However, heparin would be optimal in activation conditions requiring Ca++, as EDTA is a chelator and will deplete the Ca++. However, in cases where PMA/ionomycin is used for stimulation of whole blood, EDTA gives better cell separation between monocytes and granulocytes.
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- Is there a preference for using EDTA or heparin in Phosflow protocols?
