Is there a plasmid size restriction for DNA transfection using jetPEI™?
There is no size restriction for DNA transfection using jetPEI™. jetPEI™ is a polyamine compound which is able to compact and deliver small and large DNA molecules. The largest reported plasmid transfected in human cells using PEI is a Yeast Artificial Chromosome (YAC) of 2.3 Mb intact into human cells ( P. Marschall, N. Malik and Z. (1999) Larin Gene Therapy 6, 1634-1637). However, keep in mind that for the same amount of DNA, the gene copy number will be less for large DNA such as YACs than for smaller plasmids.
