Is there a particular flow cytometer required for the use of FlowCytomix Kits?
FlowCytomix microspheres are detectable in most commonly used flow cytometers. The flow cytometer needs to be equipped with one laser (488 nm or 532 nm) capable of detecting and distinguishing fluorescence emissions at 575 nm and far red (685 nm – 690 nm). FlowCytomix Kits have been tested for use on: EPICS XLTM / XL-MCL* Cytomics TM FC500* BD FACScan BD FACSCalibur BD FACSCanto FACSCanto II BD TM LSR I BD TM LSR II Guava EasyCyte Plus MACSQuant * For the most commonly used flow cytometers (e.g. Becton Dickinson or DakoCytomation) you can immediately start cytometer setup as below. If you run a FlowCytomix assay on a FC500 Instrument from Beckman Coulter you must ensure that the Forward Scatter (FS) measurements are collected at 1-8. To accomplish this you must insert the FS 1-8 Field Stop into place: remove the front cover to locate the FS 1-8 Field Stop. Slide the knob from the right (= default 1-19 position) to the left and push to lock in place. On the XL Series systems the EPICS X
