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Is there a limit in size for the siRNA template?

limit siRNA size Template
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Is there a limit in size for the siRNA template?

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Although there is no standard rule, SBI recommends a 19-29 nucleotide in length sequence for the siRNA template. 25-27 nucleotide siRNAs have a slightly better silencing efficiency than shorter oligos, but 19 nucleotide oligos are more commonly used.

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