Is there a defined order for aspirating and adding media to the Millicell 24 when feeding epithelial cells?
It is desirable to limit any pressure the medium may exert which may disrupt the cell contact with the filter. Therefore, the medium in the apical compartment should be present when medium is present in the basolateral compartment. The preferred order is to aspirate basolaterally first, then apically, and add fresh medium back apically first, then basolaterally.
Related Questions
- After a few hours of incubation, the behaviour of my cells changes significantly and the amount of media is reduced. What can I do against this strong evaporation?
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