Is there a checklist to ensure a valid assay run?
• Ask customer to see raw data including the standard curve fit. • Check that the OD levels for standards fall within the expected dynamic range of the assay. • Check to be sure that the correct wavelength was used in the reader. • OD values follow a trend consistent with standard order • Check that CVs are not >10%. Each laboratory must establish quality standards which determine the need for repeat testing. • Check that the standard value assignments were input correctly into the ELISA software template. • Check that the background is not elevated.
