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Is the transgene expression level affected by using two identical CMV promoters?

April 26, 2017affected CMV expression identical level transgene

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Is the transgene expression level affected by using two identical CMV promoters?

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The expression of both genes does decrease to certain degree (e.g. 3- to 5-fold lower compared with single cassette vector). But CMV is such a strong promoter and the expression levels are high enough for most experiments.