Is the thickness of the glass plates important?
Yes, the thickness of glass will affect the focal plane of the gel. As the center of the gel moves away from the focal plane of the imager, the intensity of the spots will decrease, therefore fewer spots will be seen. We recommend that the 3.4 mm-thick, low-fluorescence glass supplied for use with Amersham Biosciences equipment is used for all gels in the Ettan™ DIGE system.
