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Is the Strep-tag® system stable in the presence of imidazole?

imidazole presence STABLE system
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Is the Strep-tag® system stable in the presence of imidazole?

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Yes, the Strep-tag system tolerates up to 250 mM imidazole in the protein extract. Therefore, elution fractions from Ni-NTA resins can be directly applied on Strep-Tactin columns, which is very useful in double-tag purification protocols.

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